High-toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

Book Chapter

Book Title: Biopolymers Online

Polyamides and Complex Proteinaceous Materials

Authors:

1. Dr. Costas N. Karatzas³,
2. Nathalie Chretien⁴,
3. François Duguay⁵,
4. Annie Bellemare⁶,
5. Dr. Jiang Feng Zhou⁷,
6. Dr. Andrew Rodenhiser⁸,
7. Dr. Shafiul A. Islam⁹,
8. Carl Turcotte¹⁰,
9. Dr. Yue Huang¹¹,
10. Dr. Anthoula Lazaris¹²

Published Online: 15 JAN 2005

DOI: 10.1002/3527600035.bpol8005

Copyright © 2005 by Wiley-VCH Verlag GmbH & Co. KGaA

Jan 15, 2005  publication descriptionWiley Online Library

Publication Description:

Abstract
Introduction
Historical Outline
Production of rc-Spider Silk Proteins
Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells
Secretion of rc-Dragline Spider Silk proteins
Large-scale Production of rc-Spider Silk Proteins
Purification and Characterization of rc-Spider Silk Proteins
Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals
Generation of Transgenic Mice for the Spider Dragline Silk Genes
Mammary Gland Production of Spider Dragline Silk Proteins
Recombinant Spider Silk-based Fiber Spinning
Spin Dope Preparation, Fiber Spinning and Characterization
Fiber Post-Spinning Treatment
Perspective on the Formation of Silk Fibers and the Spinning Process
Applications of rc-Spider Silk Fibers, and Prospects
Patents
Chronological Summary of Patents
Conclusions
Acknowledgments
Keywords:
spider silk; genetic engineering; recombinant silk protein; fiber spinning;expression; mammalian cells; transgenic mice; spin dope; fibers

More Info:

http://onlinelibrary.wiley.com/doi/10.1002/3527600035.bpol8005/full

http://onlinelibrary.wiley.com/doi/10.1002/3527600035.bpol8005/abstract